Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. ©2022 Molecular Devices - All rights. Other. Contents of wells were pipetted up and down several times to mix. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 96 well plate injection time with SpectraMax® L plate reader. It also integrates with the powerful SoftMax Pro Software for. QC tools and IQOQ services. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. No tools required to assemble. 303 unable to cal dark current. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. Mode Plate Reader. Plate. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Conventional technique: subjective, time-consuming. e 3370 Costar 96 Well. 00. Categories: Plate Readers. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. The CellTiter-Glo assay was used to measure ATP. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. 5 ng per well. 3) At the bottom of the strip, click on "Show. Molecular Devices (UK) Ltd. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. We would like to show you a description here but the site won’t allow us. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. SpectraMax 250 User. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 00 . Cross-talk <0. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. HeLa. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 340 PC384. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. #C1500) Control standard endotoxin (ACC cat. An example of dual-luciferase assay results. It has a 340-750nm Spectral Range. 190. the use of a reader calibrated with primary NIST and NMI standards. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. Important things to consider when purchasing a multimode microplate reader. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. 2. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. ~ Bio-Tek ELx808 Microplate Reader $2,795. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . This study investigated the efficacy of fermented seaweed (Eucheuma cottonii) on the remediation of fluoride-contaminated soil. Fluorescence Microplate Reader Comparison Chart. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits. Silica excitation fiber, glass emission fiber. High tolerance for contaminants. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. Published Dec 16, 2020 | Updated Dec 16,. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. absorbance microplate reader GEA. (one set per assay) to clear 96-well microplates. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. 5% at 1. Measurements for every well are automatically tracked over time. Wells to read. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. Features. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. Microplate format. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…AquaMax® Microplate Washers are configurable with user interchangeable heads for both 96- and 384-well microplate washing applications, including plate or cell washing. Two high-energy xenon flash lamps: Reading capabilities. Cayman Chemical. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. validation, maintenance, and repair documentation. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Align the two slots on the bottle holder with the two pegs on the rail. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Bay Gray powder coated metal back plate, column, and base. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. This is equivalent to having 511 built-in filters,The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. 000 to 2. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Deep well blocks available in 24, 96 and 384-well formats An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. 5032338. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. x. Microplate optimization and read height optimization were performed for best results. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). 96/384-well plate formats. We would like to show you a description here but the site won’t allow us. Intuitive graphical user interface makes each assay extremely easy to set. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. Microplate Readers. 130009WMicroplate Readers. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMolecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. 96-, 384-, and 1536-well microplates. 1% in white 96- and < 0. The clear bottom plates offer the advantage of being able to examine the cells by microscopy during the course of the. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. These lab workhorses are used in drug discovery. Sort By: Show:. Compact designs make these instruments. Cell viability and cytoxicity assays. Pick the best speed, sensitivity, and price for your needs. x. Mix samples in microplate wells by user-programmable Automix feature. Materials. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. *For Corning UV plates, mean values at 260 nm ranged from 0. 2. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. 6. 0 OD. M5. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Glass microfiber: Use for cell harvesting and binding assays, liquid scintillation counting or high loading, DNA binding and purification; Melt blown polypropylene: Use for pre-filtration; Cellulose acetate: Use for low protein binding,. 00 USD. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. High-throughput, automated dynamic light scattering. We would like to show you a description here but the site won’t allow us. 00 Add to cart; Molecular Devices EMax Microplate Reader $. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. They can be read by most microplate readers. VMax . Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. External Dimensions without lid (mm). x. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution forThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Click on the crosshair and move it so that it is centered over the well image. 340 PC384. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer. 100 fmol 1536-well (8 µL) 0. Optimized for fluorescence microplate readers. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. The system features 96, 384,. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. 40266. UV absorbance detection. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Published Dec 16,. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Versa Max. The picked. $488. An industry-renowned instrument for monitoring GPCRs and ion channels. For biochemical assays, the washer removes unbound material and unreacted reagents. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Top or bottom detection is available with a simple click of a button. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. 9000-0756. No. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. ₹ 1,80,000 Get Latest Price. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. 96-, 384-, and 1536-well microplates. Temperature variation ± 0. $14,995. 96- & 384-well automated washer, multiwash™+ automated washer, 96- and 384-well plates, washer maintenance kit. Populate the following info under Description. To test the optical clarity of the various microplates at low wavelengths, one well of each plate was filled with 100 μL (96-well) or 20 μL (384-well) distilled water, and its. Page 1 of 8 . Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Bowman Dispensers. The plate reader can measure multiple readouts, including UV and. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. 2. • Easy and gentle immobilization of biotin. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. A custom 96-well plastic (polyethylene terephthalate/PET) plate is attached via epoxy to the mounted CMOS ICs, designed with a standard form factor to be compatible with high-throughput. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. 030 fmol Fluorescence Polarization (FP). Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Two high-energy xenon flash lamps: Reading capabilities. Gemini EM Reader scans. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. x. Next-generation luminescence plate reader. We would like to show you a description here but the site won’t allow us. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise:. Versa Max. Click Add to create the plate. Fluorescence Microplate Reader Comparison Chart. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Compare Tool. Uncontrolled Copy for Training and Reference Purposes Only . Site. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. x. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. Fluorescence Microplate Reader Comparison Chart. White plates reflect the light, enabling even low levels of Luminescence to be detected. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. The rate of molecular motion of Protein G slows down when it is bound to antibodies, resulting in an increase of the FP value (Figure 2). SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Loading Buffer was also added to a set of cell-free wells for background controls. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. 1. guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Instrument settings can beIntegrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. #R8195. 5 µL of each sample was pipetted into a 96-well plate for PCR. President of Molecular Devices. What are the dimensions of the plate? Code. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Optional 16-way manifold. Another consideration, says Amouretti, is plate format. Molecular Devices L-Max II Luminescence Microplate Reader. Usage/Application: Lab. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. We would like to show you a description here but the site won’t allow us. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. Compare. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. Figure 3. They are also sealable using different types of adhesive mats foils or films. View Product. x. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Integrated stacker option. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. (96- and 384-well with pipetting) 6-384 (96- and 384-well). 90349). Eight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. minutes and begin reading microplates with seven mouse clicks. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. x. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Click SoftMax Pro Software User Guide. Recertification service. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. The 6-well plates were incubated at 37ºC for 9 days before picking. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Polypropylene deep well plates, round bottom. #ABS PLUS) Methods. The IC50s by cell rounding and LysoTracker. An additional reference channel for optimizing the light intensity; Auto switch-off of the light source when not in use to extend lifetime; An automated self-check of major components at instrument start-upThe EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. 3% in white 96- and 384. Order Now. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Category 2 and 3 microplate readers use gradient heating as well. Well scanning With well scanning, multiple points within each well can be read, providing a high. Absorbance Plate Reader Comparison Chart. #N207) LAL Reagent Water (Lonza cat. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. #E010) Pyroplate 96-well microplate (ACC cat. VIS absorbance detection. Visit the supplier page or submit a quote to learn more. 984. Bioz Stars score: 86/100, based on 40 PubMed citations. CO 2 & O 2 control. style. Microplate optimization and read height optimization were performed for best results. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. Can read 96 well plates (optional 384 well). Or scan up to 384 wells at any time to determine the best assay conditions. Compare specifications and request a quote from leading suppliers. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. 0% ± 0. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. The advanced design of the Sunrise plate reader includes. No additional manuals are available. 003 fmol 0. 5L) MultiWash+. 1 x plates; Instrument dimensions. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Dark blue indicates well will be read. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. Microplate Readers include the M3, M4, M5, and M5e models. 0. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). % transmittance of plates used in a plate reader. Plate Shaking. Molecular Spectroscopy Consumables & Accessories Thermal Analysis. Janet got excellent results using them and they are only ~$1. In addition, users can define individual reagents and concentrations to be delivered to each well. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Turn the instrument around so that the back of the instrument is. x. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Use standard plate unless you see your type listed. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. PURPOSE. The Versamax Microplate Reader is built around a grating monochromator. 1. x. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. 7°C (gradient heating design). QuickSync. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. Three kits covering a wide range of dsDNA concentrations. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. 4 (Molecular Devices cat. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. 1 mL/well of media. All trademarks used herein are the property of. XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). Select up to 3 products. Includes PC and software. Quickly image a variety of plate formats and cell types - Image a 96-well plate in under two minutes. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. 000 OD ± 1. Sample by eye within . Multimode microplate reader equipped with latest detections like fluorescence,. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0.